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KMID : 0366220070420040375
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Korean Journal of Hematology
2007 Volume.42 No. 4 p.375 ~ p.381
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Immunomodulation Effect of the Allogeneic Cellular Components after Transfusion
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Kim Jung-Jin
Yoo Hyo-Joo
Kwon Young-Joo
Chung Nack-Gyun
Cho Bin
Kim Tai-Gyu
Kim Hack-Ki
Han Chi-Hwa
Jeong Chang-Mo
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Abstract
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Background: Blood transfusion is important for life saving treatment in many patients with tolerable adverse effects. Some data suggest that transfusions might cause an increased risk for post-operative infections and a higher relapse or mortality rate in cancer patients. We investigated whether immune dysfunction might result after transfusions from the cellular components.
Methods: We studied 5-week-old mice BALB/c (H-2d, donor), C3H/He (H-2k, recipient), and C57/BL (H-2b, third party). We obtained irradiated spleen cells (SP) from the BALB/c or C57/BL, and injected them into the C3H/He with intraperitoneal IL-2 administration. After 24 hours, we obtained bone marrow (BM), thymus and SP. We identified mixed lymphocyte proliferation (MLR) by the BrdU method and we used irradiated BALB/c SP, as a stimulator for that trial. For the analysis of immune cells, we analyzed the cell surface markers from each organ. For cytokines, we identified TNF-?, IFN-?, TGF-?, and IL-10 by ELISA from the supernatant of the MLR.
Results: The cell proliferation decreased according to specific H-2 complexes. There were increased CD4+CD25+ cells in the thymus. For the paracrine effects, the B-C3H SP showed ratio-dependent inhibitory effects, although the C-C3H SP inhibited some cell proliferation. There was no difference in the IFN-?, TNF-? and TGF-? between the control and experimental groups. However, IL-10 was higher in the 1：10 ratio mixture in the control and transfused SP compared to the other groups.
Conclusion: The results of this study suggested that the cellular components in transfusions might contribute to the immune regulatory effects by CD4+CD25+ cells after 24 hours. (Korean J Hematol 2007;42:375-381.)
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KEYWORD
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Transfusion, Immunomodulation, IL-10, CD4+CD25+ cells
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